Standardization in AAV gene therapy

Optimize your workflow in AAV gene therapy research and development!

Customized adeno-associated virus (AAV) vectors are universal gene shuttles in human cells and powerful tools in gene therapy. Recent progress in research and development has demonstrated that AAV gene delivery is safe and effective.
The development of functional AAV gene shuttle for effective clinical approaches mainly involves three intricate steps: a) Generation of a tissue-specific plasmid DNA, b) engineering of the appropriate capsid and packaging of the AAV vector, and finally, c) accurate quantification of recombinant AAV particles (AAV titer).

In order to optimize your workflow and increase the experimental success of your AAV vector system, PlasmidFactory, Sirion Biotech and PROGEN offer compatible and established products that cover the entire process – from plasmid design to the determination of the effective transduction dose.

Meet us at ASGCT 2019

MANUFACTURING OF AAV PLASMIDS BY PLASMIDFACTORY

Helper & Packaging and vector plasmids for AAV production
• Cost-effective & reliable 2-plasmid-system
In Stock Service for immediate delivery
• Wide range of AAV plasmids of the pDG/ pDP family for different AAV serotypes In Stock
• AAV minicircles for high productivity & correct packaging
• Contract manufacturing of individual ITR-containing vector plasmids and minicircles
High Quality Grade for GMP production of viral vectors

Contact PlasmidFactory for more information

Check PlasmidFactory's In Stock Service page


ENGINEERING & MANUFACTURING OF THE AAV VECTOR                        BY SIRION BIOTECH

Custom AAV engineering & production
• Custom engineering and pilot-manufacturing of 5 x 1012 – 1 x 1014 genetic copies (GC)
• Scale up of preclinical processes and GMP production
• AAV serotypes 1, 1/2, 2, 3, 4, 5, 6 & custom capsid modifications

Contact Sirion for more information

Check Sirion's page on engineered AAV vectors


QUANTIFICATION OF AAV TITERS BY PROGEN

Determination of recombinant AAV particles by titration ELISA
• Dependable & reproducible AAV titer quantification
• Specific recognition of intact AAV particles
• Available for AAV serotypes 1, 2, 5, 6, 8, 9
• Published & validated assays for standardized approaches
• Damage-free AAV purification with OptiPrepTM

Contact PROGEN for more information

Check PROGEN's AAV page

Shop PROGEN's unique range of AAV quantification ELISAs!


AAV VECTOR QUALITY CONTROL BY GENEWERK 

Quantification of impurities and integration site analysis
• Vector integration site and clonality analysis
• Identification of potential oncogenes cross over from vector production system
• Identification and quantification of nucleotide impurities by NGS
• Vector integrity and irregular encapsidation

Contact GeneWerk for more information

Check GeneWerk's AAV Vector Quality Control